Using the newly microfluidic biosensor for carcinoembryonic antigen detection

Chia Hsien Yeh, Kuan Feng Su, Yu Cheng Lin, Pi Lan Shen

Research output: Contribution to journalConference article


A newly microfluidic biosensor was successfully utilized the microchannel, the sandwich immunoassay model, and a parallel electrode to detect the electrical signal of various carcinoembryonic antigen (CEA) concentrations. A microfluidic biosensor was based on AuNPs by using the specific binding of antibody to reduce the impedance measurement. The impedance variation can be easily detected with a LCR meter, and we observed an obvious and stable difference in impedance signals at 8 min. Moreover, a better linearity for impedance and CEA concentration in the frequency effect (100 to 1 MHz) was observed at 100 Hz. Finally, the detectable limit for CEA concentration was 0.1 ng/mL with 1 μg/mL of anti-CEA antibody and the 50X of the anti-CEA antibody-colloidal gold conjugates. Therefore, the developed microfluidic biosensor has advantages including smaller sample volume (10 μL, while the commercial product is 100 μL) and a rapidly quantitative measurement, and works with smaller detectable concentration (0.1 ng/mL) than other existing commercial products whose best limit of detection is 5 ng/mL.

Original languageEnglish
Article number6985392
Pages (from-to)1866-1868
Number of pages3
JournalProceedings of IEEE Sensors
Issue numberDecember
Publication statusPublished - 2014 Dec 12
Event13th IEEE SENSORS Conference, SENSORS 2014 - Valencia, Spain
Duration: 2014 Nov 22014 Nov 5

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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