TY - GEN
T1 - Utilizing genes and quantum dots to verify the design and experimental performance of the electroporation biochip for transgenic zebrafishes
AU - Hung, Ching Yuan
AU - Huang, Hao Hsuan
AU - Su, Kai Chun
AU - Wang, Yu Tsung
AU - Lin, Yu Cheng
PY - 2005
Y1 - 2005
N2 - This study was focused on the design and experimental verification of an electroporation (EP) biochip for transgenic zebrafishes (Danio rerio). For generating suitable pulses, a lab-built circuit was designed to provide suitable voltages (0 - 200 V) and produce almost 1,000-V/cm electric field in the transgenic zebrafish experiments. In addition, a novel biochip composed of biocompatible electrodes, holder device, and in-vitro electroporation chip was fabricated by micro electromechanical system (MEMS) technology. Modular design would aid replacing each component conveniently and rapidly. (CdSe)ZnS quantum dots, QDs, genes coding for green fluorescence protein, GFP, and trypan-blue dye were used to verify the successful transfection to the zebrafish embryos. The experimental results showed that the delivery rate was up to 62% utilizing an EP biochip to deliver trypan-blue dye, QDs or GFP genes into zebrafish embryos. This study demonstrated that the gene transfer could be easily accomplished by simply-designed circuit and quickly observed by quantum dots, and it will be helpful for applications to the elaboration of aquaculture fishery in the future.
AB - This study was focused on the design and experimental verification of an electroporation (EP) biochip for transgenic zebrafishes (Danio rerio). For generating suitable pulses, a lab-built circuit was designed to provide suitable voltages (0 - 200 V) and produce almost 1,000-V/cm electric field in the transgenic zebrafish experiments. In addition, a novel biochip composed of biocompatible electrodes, holder device, and in-vitro electroporation chip was fabricated by micro electromechanical system (MEMS) technology. Modular design would aid replacing each component conveniently and rapidly. (CdSe)ZnS quantum dots, QDs, genes coding for green fluorescence protein, GFP, and trypan-blue dye were used to verify the successful transfection to the zebrafish embryos. The experimental results showed that the delivery rate was up to 62% utilizing an EP biochip to deliver trypan-blue dye, QDs or GFP genes into zebrafish embryos. This study demonstrated that the gene transfer could be easily accomplished by simply-designed circuit and quickly observed by quantum dots, and it will be helpful for applications to the elaboration of aquaculture fishery in the future.
UR - http://www.scopus.com/inward/record.url?scp=33748901632&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748901632&partnerID=8YFLogxK
U2 - 10.1109/ICMECH.2005.1529364
DO - 10.1109/ICMECH.2005.1529364
M3 - Conference contribution
AN - SCOPUS:33748901632
SN - 0780389980
SN - 9780780389984
T3 - Proceedings of the 2005 IEEE International Conference on Mechatronics, ICM '05
SP - 799
EP - 804
BT - Proceedings of the 2005 IEEE International Conference on Mechatronics, ICM '05
T2 - 2005 IEEE International Conference on Mechatronics, ICM '05
Y2 - 10 July 2005 through 12 July 2005
ER -