@article{402b4bc51dda442ea4241b167e09df96,
title = "UvrY is required for the full virulence of Aeromonas dhakensis",
abstract = "Aeromonas dhakensis is an emerging human pathogen which causes fast and severe infections worldwide. Under the gradual pressure of lacking useful antibiotics, finding a new strategy against A. dhakensis infection is urgent. To understand its pathogenesis, we created an A. dhakensis AAK1 mini-Tn10 transposon library to study the mechanism of A. dhakensis infection. By using a Caenorhabditis elegans model, we established a screening platform for the purpose of identifying attenuated mutants. The uvrY mutant, which conferred the most attenuated toxicity toward C. elegans, was identified. The uvrY mutant was also less virulent in C2C12 fibroblast and mice models, in line with in vitro results. To further elucidate the mechanism of UvrY in controlling the toxicity in A. dhakensis, we conducted a transcriptomic analysis. The RNAseq results showed that the expression of a unique hemolysin ahh1 and other virulence factors were regulated by UvrY. Complementation of Ahh1, one of the most important virulence factors, rescued the pore-formation phenotype of uvrY mutant in C. elegans; however, complementation of ahh1 endogenous promoter-driven ahh1 could not produce Ahh1 and rescue the virulence in the uvrY mutant. These findings suggest that UvrY is required for the expression of Ahh1 in A. dhakensis. Taken together, our results suggested that UvrY controls several different virulence factors and is required for the full virulence of A. dhakensis. The two-component regulator UvrY therefore a potential therapeutic target which is worthy of further study.",
author = "Chen, {Yi Wei} and Yeh, {Wen Hsuan} and Tang, {Hung Jen} and Chen, {Jenn Wei} and Shu, {Hung Yu} and Su, {Yu Chen} and Wang, {Sin Tian} and Kuo, {Cheng Ju} and Chuang, {Yin Ching} and Chen, {Chi Chung} and Ko, {Wen Chien} and Chen, {Chang Shi} and Chen, {Po Lin}",
note = "Funding Information: The C. elegans wild-type Bristol N2 strain and PD4793 mIs10[myo-2p::GFP + pes-10p::GFP + gut-promoter::GFP] worms were provided by the Caenorhabditis Genetics Center (CGC), which is supported by the National Institutes of Health – Office of Research Infrastructure Programs (P40 OD010440). The animals were maintained on Nematode Growth Medium (NGM) with E. coli strain OP50 as the normal food source. Funding Information: This work was supported by the Ministry of Science and Technology, Taiwan [MOST 107-2628-B-006-003] to CSC; Ministry of Science and Technology, Taiwan [MOST 108-2628-B-006-012] to PLC; National Cheng Kung University Hospital [NCKUH-10802036] to PLC. The NGS and mapping of RNAseq were performed using the system provided by the Bioinformatics Core at National Cheng Kung University, supported by the Ministry of Science and Technology, Taiwan. We would like to thank for providing experimental space and facilities by the Diagnostic Microbiology and Antimicrobial Resistance Laboratory, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. We also thank Savana Moore, who is a native speaker of English and an expert in editing science research for the Foreign Language Center at National Cheng Kung University. Funding Information: The NGS and mapping of RNAseq were performed using the system provided by the Bioinformatics Core at National Cheng Kung University, supported by the Ministry of Science and Technology, Taiwan. We would like to thank for providing experimental space and facilities by the Diagnostic Microbiology and Antimicrobial Resistance Laboratory, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. We also thank Savana Moore, who is a native speaker of English and an expert in editing science research for the Foreign Language Center at National Cheng Kung University. Publisher Copyright: {\textcopyright} 2020, {\textcopyright} 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",
year = "2020",
month = jan,
day = "1",
doi = "10.1080/21505594.2020.1768339",
language = "English",
volume = "11",
pages = "502--520",
journal = "Virulence",
issn = "2150-5594",
publisher = "Landes Bioscience",
number = "1",
}