Vaccination with three inactivated pathogens of cobia (Rachycentron canadum) stimulates protective immunity

John Han You Lin, Tzong Yueh Chen, Ming Shyan Chen, Huey En Chen, Ruey Liang Chou, Tzyy Ing Chen, Mao Sen Su, Huey Lang Yang

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Cobia (Rachycentron canadum), a warm water fish recently commercially cultured in Taiwan, has encountered severe mortalities with survival rates often below 20%. The major causative pathogens were Vibrio alginolyticus, Vibrio parahaemolyticus and Photobacterium damselae subsp. piscicida. We prepared a combined three inactivated bacterins antigen preparation and vaccinated cobia. This mixture of bacterins was safe, and the immune response in cobia stimulated specific antibody in 1 week that remained for at least 6 weeks until the end of the aquarium trial. Its efficacy in protecting fish was evaluated in aquarium and field trials. In the aquarium challenge, the vaccine gave a relative percentage survival of 93.8%, 91.1% and 84.7% after challenge with V. alginolyticus, V. parahaemolyticus and P. damselae subsp. piscicida, respectively. In two farm trials using two batches of fish from different hatcheries, one vaccination gave a survival rate of 86-92%. A single vaccination of three combined inactivated bacteria into cobia elicited specific antibodies, and protected fish in both the laboratory aquarium challenge and a farm trial.

Original languageEnglish
Pages (from-to)125-132
Number of pages8
Issue number1-4
Publication statusPublished - 2006 May 31

All Science Journal Classification (ASJC) codes

  • Aquatic Science


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