VIBRIO HARVEYI-SPECIFIC BINDING DOWN SYNDROME CELL ADHESION MOLECULE, METHOD FOR IDENTIFICATION THEREOF AND USE THEREOF

Han-Ching Wang (Inventor)

Research output: Patent

Abstract

The present invention relates to a Vibrio harveyi-specific binding Dscam of shrimps, a method for identification thereof and a use thereof. Primarily, Dscam cDNAs are prepared from V. harveyi-challenged shrimps. A database is established with various Dscam isoforms therein by means of numerous cloning and sequencing. After a phylogenetic analysis, a plurality of candidate Dscam isoforms is selected. Each recombinant protein of candidate Dscam isoforms is expressed through an expression system. Finally, the binding affinity between V. harveyi and each recombinant protein of candidate Dscam isoforms is measured to further selection of a V. harveyi-specific binding Dscam and the amino acid sequence thereof. Accordingly, the Dscam can be specifically binding to V. harveyi for eliminating or reducing V. harveyi.
Original languageEnglish
Patent number9328151
Publication statusPublished - 2015 Feb 12

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Vibrio
Cell Adhesion Molecules
Down Syndrome
Protein Isoforms
Recombinant Proteins
Organism Cloning
Amino Acid Sequence
Complementary DNA
Databases

Cite this

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title = "VIBRIO HARVEYI-SPECIFIC BINDING DOWN SYNDROME CELL ADHESION MOLECULE, METHOD FOR IDENTIFICATION THEREOF AND USE THEREOF",
abstract = "The present invention relates to a Vibrio harveyi-specific binding Dscam of shrimps, a method for identification thereof and a use thereof. Primarily, Dscam cDNAs are prepared from V. harveyi-challenged shrimps. A database is established with various Dscam isoforms therein by means of numerous cloning and sequencing. After a phylogenetic analysis, a plurality of candidate Dscam isoforms is selected. Each recombinant protein of candidate Dscam isoforms is expressed through an expression system. Finally, the binding affinity between V. harveyi and each recombinant protein of candidate Dscam isoforms is measured to further selection of a V. harveyi-specific binding Dscam and the amino acid sequence thereof. Accordingly, the Dscam can be specifically binding to V. harveyi for eliminating or reducing V. harveyi.",
author = "Han-Ching Wang",
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N2 - The present invention relates to a Vibrio harveyi-specific binding Dscam of shrimps, a method for identification thereof and a use thereof. Primarily, Dscam cDNAs are prepared from V. harveyi-challenged shrimps. A database is established with various Dscam isoforms therein by means of numerous cloning and sequencing. After a phylogenetic analysis, a plurality of candidate Dscam isoforms is selected. Each recombinant protein of candidate Dscam isoforms is expressed through an expression system. Finally, the binding affinity between V. harveyi and each recombinant protein of candidate Dscam isoforms is measured to further selection of a V. harveyi-specific binding Dscam and the amino acid sequence thereof. Accordingly, the Dscam can be specifically binding to V. harveyi for eliminating or reducing V. harveyi.

AB - The present invention relates to a Vibrio harveyi-specific binding Dscam of shrimps, a method for identification thereof and a use thereof. Primarily, Dscam cDNAs are prepared from V. harveyi-challenged shrimps. A database is established with various Dscam isoforms therein by means of numerous cloning and sequencing. After a phylogenetic analysis, a plurality of candidate Dscam isoforms is selected. Each recombinant protein of candidate Dscam isoforms is expressed through an expression system. Finally, the binding affinity between V. harveyi and each recombinant protein of candidate Dscam isoforms is measured to further selection of a V. harveyi-specific binding Dscam and the amino acid sequence thereof. Accordingly, the Dscam can be specifically binding to V. harveyi for eliminating or reducing V. harveyi.

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