TY - JOUR
T1 - Viral circular RNA–encoded protein, ceVP28, divulges an antiviral response in invertebrates
AU - Limkul, Sirawich
AU - Phiwthong, Tannatorn
AU - Wanvimonsuk, Supitcha
AU - Seabkongseng, Tuangrak
AU - Aunkam, Phirom
AU - Jaree, Phattarunda
AU - Luangtrakul, Waruntorn
AU - Mahanil, Kanjana
AU - Teamtisong, Kamonluck
AU - Tittabutr, Panlada
AU - Teaumroong, Neung
AU - Sarnow, Peter
AU - Wang, Han Ching
AU - Somboonwiwat, Kunlaya
AU - Boonchuen, Pakpoom
N1 - Publisher Copyright:
Copyright © 2025 the Author(s).
PY - 2025/2/25
Y1 - 2025/2/25
N2 - Invertebrates mostly use innate immunity to counteract pathogenic infections. In this study, shrimp was used as a model organism to explore the functions of circular RNAs (circRNAs) derived from white spot syndrome virus (WSSV). We identified four viral circRNAs, termed circWSSV147, circWSSV326, circWSSV458, and circVP28, from transcriptomic data of WSSV-infected shrimp. CircVP28, which contains an internal ribosome entry site, was further characterized to determine its potential as a template for protein translation. We observed the presence of a truncated, circRNA-encoded VP28 (ceVP28) in infected shrimp. Both ceVP28 and its parental counterpart, VP28, share the same host cell binding partner Rab7, which is a host receptor for WSSV. Coadministration of recombinant ceVP28 protein and WSSV to penaeid shrimps reduced both viral copy numbers and mortality upon WSSV challenges. These findings uncovered a host defense mechanism by which a protein encoded by a viral circRNA modulates virus–receptor interactions, resulting in blocking of viral entry.
AB - Invertebrates mostly use innate immunity to counteract pathogenic infections. In this study, shrimp was used as a model organism to explore the functions of circular RNAs (circRNAs) derived from white spot syndrome virus (WSSV). We identified four viral circRNAs, termed circWSSV147, circWSSV326, circWSSV458, and circVP28, from transcriptomic data of WSSV-infected shrimp. CircVP28, which contains an internal ribosome entry site, was further characterized to determine its potential as a template for protein translation. We observed the presence of a truncated, circRNA-encoded VP28 (ceVP28) in infected shrimp. Both ceVP28 and its parental counterpart, VP28, share the same host cell binding partner Rab7, which is a host receptor for WSSV. Coadministration of recombinant ceVP28 protein and WSSV to penaeid shrimps reduced both viral copy numbers and mortality upon WSSV challenges. These findings uncovered a host defense mechanism by which a protein encoded by a viral circRNA modulates virus–receptor interactions, resulting in blocking of viral entry.
UR - https://www.scopus.com/pages/publications/85218932441
UR - https://www.scopus.com/pages/publications/85218932441#tab=citedBy
U2 - 10.1073/pnas.2321707122
DO - 10.1073/pnas.2321707122
M3 - Article
C2 - 39964719
AN - SCOPUS:85218932441
SN - 0027-8424
VL - 122
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
M1 - e2321707122
ER -