Virulence diversity among bacteremic aeromonas isolates: Ex Vivo, animal, and clinical evidences

Chen Po-Lin, Wu Chi-Jung, Tsai Pei-Jane, Tang Hung-Jen, Chuang Yin-Ching, Lee Nan-Yao, Lee Ching-Chi, Li Chia-Wen, Li Ming-Chi, Chen Hung Wen Tsai Chi-Chung, Ou Chun-Chun, Chen Chang-Shi, Ko Wen-Chien

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Conclusions/Significance: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.

Methodology/Principal Findings: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5% vs. 0%, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values〈0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values〈0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values 〈0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.

The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.

Original languageEnglish
Article numbere111213
JournalPloS one
Volume9
Issue number11
DOIs
Publication statusPublished - 2014 Nov 6

Fingerprint

Aeromonas
Virulence
Animals
virulence
Cells
DNA sequences
Virulence Factors
Fibroblasts
Cytotoxicity
animals
Blood
Survival Rate
Genes
Aeromonas veronii
survival rate
cell lines
Aeromonas punctata
Cell Line
sepsis (infection)
bacteremia

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Po-Lin, Chen ; Chi-Jung, Wu ; Pei-Jane, Tsai ; Hung-Jen, Tang ; Yin-Ching, Chuang ; Nan-Yao, Lee ; Ching-Chi, Lee ; Chia-Wen, Li ; Ming-Chi, Li ; Chi-Chung, Chen Hung Wen Tsai ; Chun-Chun, Ou ; Chang-Shi, Chen ; Wen-Chien, Ko. / Virulence diversity among bacteremic aeromonas isolates : Ex Vivo, animal, and clinical evidences. In: PloS one. 2014 ; Vol. 9, No. 11.
@article{c5b2f14a50724b0d8d6d18e909b2268b,
title = "Virulence diversity among bacteremic aeromonas isolates: Ex Vivo, animal, and clinical evidences",
abstract = "Conclusions/Significance: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.Methodology/Principal Findings: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5{\%} vs. 0{\%}, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values〈0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values〈0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values 〈0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.",
author = "Chen Po-Lin and Wu Chi-Jung and Tsai Pei-Jane and Tang Hung-Jen and Chuang Yin-Ching and Lee Nan-Yao and Lee Ching-Chi and Li Chia-Wen and Li Ming-Chi and Chi-Chung, {Chen Hung Wen Tsai} and Ou Chun-Chun and Chen Chang-Shi and Ko Wen-Chien",
year = "2014",
month = "11",
day = "6",
doi = "10.1371/journal.pone.0111213",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

Virulence diversity among bacteremic aeromonas isolates : Ex Vivo, animal, and clinical evidences. / Po-Lin, Chen; Chi-Jung, Wu; Pei-Jane, Tsai; Hung-Jen, Tang; Yin-Ching, Chuang; Nan-Yao, Lee; Ching-Chi, Lee; Chia-Wen, Li; Ming-Chi, Li; Chi-Chung, Chen Hung Wen Tsai; Chun-Chun, Ou; Chang-Shi, Chen; Wen-Chien, Ko.

In: PloS one, Vol. 9, No. 11, e111213, 06.11.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Virulence diversity among bacteremic aeromonas isolates

T2 - Ex Vivo, animal, and clinical evidences

AU - Po-Lin, Chen

AU - Chi-Jung, Wu

AU - Pei-Jane, Tsai

AU - Hung-Jen, Tang

AU - Yin-Ching, Chuang

AU - Nan-Yao, Lee

AU - Ching-Chi, Lee

AU - Chia-Wen, Li

AU - Ming-Chi, Li

AU - Chi-Chung, Chen Hung Wen Tsai

AU - Chun-Chun, Ou

AU - Chang-Shi, Chen

AU - Wen-Chien, Ko

PY - 2014/11/6

Y1 - 2014/11/6

N2 - Conclusions/Significance: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.Methodology/Principal Findings: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5% vs. 0%, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values〈0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values〈0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values 〈0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.

AB - Conclusions/Significance: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.Methodology/Principal Findings: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5% vs. 0%, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values〈0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values〈0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values 〈0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.

UR - http://www.scopus.com/inward/record.url?scp=84909989293&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84909989293&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0111213

DO - 10.1371/journal.pone.0111213

M3 - Article

C2 - 25375798

AN - SCOPUS:84909989293

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e111213

ER -