Virus-induced gene silencing unravels multiple transcription factors involved in floral growth and development in Phalaenopsis orchids

Ming Hsien Hsieh, Zhao Jun Pan, Pei Han Lai, Hsiang Chia Lu, Hsin Hung Yeh, Chia Chi Hsu, Wan Lin Wu, Mei Chu Chung, Shyh Shyan Wang, Wen Huei Chen, Hong Hwa Chen

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Orchidaceae, one of the largest angiosperm families, has significant commercial value. Isolation of genes involved in orchid floral development and morphogenesis, scent production, and colouration will advance knowledge of orchid flower formation and facilitate breeding new varieties to increase the commercial value. With high-throughput virus-induced gene silencing (VIGS), this study identified five transcription factors involved in various aspects of flower morphogenesis in the orchid Phalaenopsis equestris. These genes are PeMADS1, PeMADS7, PeHB, PebHLH, and PeZIP. Silencing PeMADS1 and PebHLH resulted in reduced flower size together with a pelaloid column containing petal-like epidermal cells and alterations of epidermal cell arrangement in lip lateral lobes, respectively. Silencing PeMADS7, PeHB, and PeZIP alone resulted in abortion of the first three fully developed flower buds of an inflorescence, which indicates the roles of the genes in late flower development. Furthermore, double silencing PeMADS1 and PeMADS6, C-and B-class MADS-box genes, respectively, produced a combinatorial phenotype with two genes cloned in separate vectors. Both PeMADS1 and PeMADS6 are required to ensure the normal development of the lip and column as well as the cuticle formation on the floral epidermal cell surface. Thus, VIGS allows for unravelling the interaction between two classes of MADS transcription factors for dictating orchid floral morphogenesis.

Original languageEnglish
Pages (from-to)3869-3884
Number of pages16
JournalJournal of experimental botany
Volume64
Issue number12
DOIs
Publication statusPublished - 2013 Sep

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Fingerprint

Dive into the research topics of 'Virus-induced gene silencing unravels multiple transcription factors involved in floral growth and development in Phalaenopsis orchids'. Together they form a unique fingerprint.

Cite this