Virus Purification by bead-based immunoreaction and rapid electrokinetic patterning

A. Y. Chen, Han-Sheng Chuang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Purification of virus from blood is challenging because of interference of numerous impurity. Ultracentrifugation is a conventional practice, but time consumption and required large sample volume. Depends on the antibody specificity and manipulation techniques advance, the three steps process was proposed to purify the virus. Using bead-based immunoreaction to capture the virus, and sorting particles by rapid electrokinetic patterning (REP), then separating the droplet into two individual ones. The concept of bead-based immunoreaction was verified by gold nanoparticles (AuNPs) and Enterovirus 71 (EV71). In the future, the droplet separation will be accomplished by changing the surface property of the chips.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1535-1536
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16-10-0916-10-13

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'Virus Purification by bead-based immunoreaction and rapid electrokinetic patterning'. Together they form a unique fingerprint.

  • Cite this

    Chen, A. Y., & Chuang, H-S. (2016). Virus Purification by bead-based immunoreaction and rapid electrokinetic patterning. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1535-1536). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.