Virus replication and cytokine production in dengue virus-infected human B lymphocytes

Yu Wen Lin, Kuan Ju Wang, Huan Yao Lei, Yee Shin Lin, Trai Ming Yeh, Hsiao Sheng Liu, Ching Chuan Liu, Shun Hua Chen

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80 Citations (Scopus)


Dengue virus (DV) replication, antibody-enhanced viral infection, and cytokine responses of human primary B lymphocytes (cells) were characterized and compared with those of monocytes. The presence of a replication template (negative-strand RNA intermediate), viral antigens including core and nonstructural proteins, and increasing amounts of virus with time postinfection indicated that DV actively replicated in B cells. Virus infection' also induced B cells to produce interleukin-6 and tumor necrosis factor alpha, which have been previously implicated in virus pathogenesis. In addition, a heterologous antibody was able to enhance both virus and cytokine production in B cells. Furthermore, the levels of virus replication, antibody-enhanced virus replication, and cytokine responses observed in B cells were not statistically different from those in monocytes. These results suggest that B cells may play an important role in DV pathogenesis.

Original languageEnglish
Pages (from-to)12242-12249
Number of pages8
JournalJournal of Virology
Issue number23
Publication statusPublished - 2002 Dec

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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