Visualization of gram-positive bacterial pili

Chungyu Chang, I. Hsiu Huang, Antoni P.A. Hendrickx, Hung Ton-That

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Citations (Scopus)


Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.

Original languageEnglish
Title of host publicationBacterial Cell Surfaces
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages19
ISBN (Print)9781627032445
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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