Visualization of gram-positive bacterial pili

Chungyu Chang, I. Hsiu Huang, Antoni P.A. Hendrickx, Hung Ton-That

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Citations (Scopus)

Abstract

Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.

Original languageEnglish
Title of host publicationBacterial Cell Surfaces
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages77-95
Number of pages19
ISBN (Print)9781627032445
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume966
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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