Vitamin D Can Ameliorate Chlorhexidine Gluconate-Induced Peritoneal Fibrosis and Functional Deterioration through the Inhibition of Epithelial-to-Mesenchymal Transition of Mesothelial Cells

Yi Che Lee, Shih Yuan Hung, Hung Hsiang Liou, Tsun Mei Lin, Chu Hung Tsai, Sheng Hsiang Lin, Yau Sheng Tsai, Min Yu Chang, Hsi Hao Wang, Li Chun Ho, Yi Ting Chen, Ching Fang Wu, Ho Ching Chen, Hsin Pao Chen, Kuang Wen Liu, Chih I. Chen, Kuan Min She, Hao Kuang Wang, Chi Wei Lin, Yuan Yow Chiou

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11 Citations (Scopus)

Abstract

Background. Peritoneal dialysis (PD) can induce fibrosis and functional alterations in PD patients' peritoneal membranes, due to long-term unphysiological dialysate exposure, partially occurring via triggering of epithelial-to-mesenchymal transition (EMT) in peritoneal mesothelial cells (MCs). Vitamin D can ameliorate these negative effects; however, the mechanism remains unexplored. Therefore, we investigated its possible links to MCs EMT inhibition. Methods. Peritoneal fibrosis was established in Sprague-Dawley rats by chlorhexidine gluconate (CG) intraperitoneal injection for 21 days, with and without 1,25(OH)treatment. Morphological and functional evaluation and western blot analysis of EMT marker were performed upon peritoneum tissue. In vitro study was also performed in a primary human peritoneal MC culture system; MCs were incubated with transforming growth factor-β1 (TGF-β1) in the absence or presence of 1,25(OH) EMT marker expression, migration activities, and cytoskeleton redistribution of MCs were determined. Results. 1,25(OH)ameliorated CG-induced morphological and functional deterioration in animal model, along with CG-induced upregulation of -SMA and downregulation of E-cadherin expression. Meanwhile, 1,25(OH)also ameliorated TGF-β1-induced decrease in E-cadherin expression, increase in Snai1 and -SMA expression, intracellular F-actin redistribution, and migration activity in vitro. Conclusion. 1,25(OH)can ameliorate CG-induced peritoneal fibrosis and attenuate functional deterioration through inhibiting MC EMT.

Original languageEnglish
Article number595030
JournalBioMed research international
Volume2015
DOIs
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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