Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy

Junjie Yao, Chih-Hsien Huang, Catherine Martel, Konstantin I. Maslov, Lidai Wang, Joon Mo Yang, Liang Gao, Gwendalyn Randolph, Jun Zou, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2013
DOIs
Publication statusPublished - 2013 May 28
EventPhotons Plus Ultrasound: Imaging and Sensing 2013 - San Francisco, CA, United States
Duration: 2013 Feb 32013 Feb 5

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8581
ISSN (Print)1605-7422

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2013
CountryUnited States
CitySan Francisco, CA
Period13-02-0313-02-05

Fingerprint

Photoacoustic microscopy
Micro-Electrical-Mechanical Systems
photoacoustic microscopy
microelectromechanical systems
MEMS
Microscopy
Mirrors
mirrors
Scanning
scanning
Water
water
Imaging techniques
mice
Acoustics
Gold coatings
gold coatings
MOEMS
microoptoelectromechanical systems
Evans Blue

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Yao, J., Huang, C-H., Martel, C., Maslov, K. I., Wang, L., Yang, J. M., ... Wang, L. V. (2013). Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. In Photons Plus Ultrasound: Imaging and Sensing 2013 [858127] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8581). https://doi.org/10.1117/12.2005669
Yao, Junjie ; Huang, Chih-Hsien ; Martel, Catherine ; Maslov, Konstantin I. ; Wang, Lidai ; Yang, Joon Mo ; Gao, Liang ; Randolph, Gwendalyn ; Zou, Jun ; Wang, Lihong V. / Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. Photons Plus Ultrasound: Imaging and Sensing 2013. 2013. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{6a445339d9764fed990f408097aee35b,
title = "Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy",
abstract = "By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.",
author = "Junjie Yao and Chih-Hsien Huang and Catherine Martel and Maslov, {Konstantin I.} and Lidai Wang and Yang, {Joon Mo} and Liang Gao and Gwendalyn Randolph and Jun Zou and Wang, {Lihong V.}",
year = "2013",
month = "5",
day = "28",
doi = "10.1117/12.2005669",
language = "English",
isbn = "9780819493507",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Photons Plus Ultrasound",

}

Yao, J, Huang, C-H, Martel, C, Maslov, KI, Wang, L, Yang, JM, Gao, L, Randolph, G, Zou, J & Wang, LV 2013, Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. in Photons Plus Ultrasound: Imaging and Sensing 2013., 858127, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, San Francisco, CA, United States, 13-02-03. https://doi.org/10.1117/12.2005669

Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. / Yao, Junjie; Huang, Chih-Hsien; Martel, Catherine; Maslov, Konstantin I.; Wang, Lidai; Yang, Joon Mo; Gao, Liang; Randolph, Gwendalyn; Zou, Jun; Wang, Lihong V.

Photons Plus Ultrasound: Imaging and Sensing 2013. 2013. 858127 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8581).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy

AU - Yao, Junjie

AU - Huang, Chih-Hsien

AU - Martel, Catherine

AU - Maslov, Konstantin I.

AU - Wang, Lidai

AU - Yang, Joon Mo

AU - Gao, Liang

AU - Randolph, Gwendalyn

AU - Zou, Jun

AU - Wang, Lihong V.

PY - 2013/5/28

Y1 - 2013/5/28

N2 - By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

AB - By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

UR - http://www.scopus.com/inward/record.url?scp=84878082257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878082257&partnerID=8YFLogxK

U2 - 10.1117/12.2005669

DO - 10.1117/12.2005669

M3 - Conference contribution

SN - 9780819493507

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Photons Plus Ultrasound

ER -

Yao J, Huang C-H, Martel C, Maslov KI, Wang L, Yang JM et al. Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. In Photons Plus Ultrasound: Imaging and Sensing 2013. 2013. 858127. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2005669