TY - GEN
T1 - Water-soluble (MUA-coated) Quantum dots
T2 - Microfluidics, BioMEMS, and Medical Microsystems V
AU - Yang, Chih Hui
AU - Huang, Keng Shiang
AU - Lee, Sheng Ji
AU - Lin, Kuo Chin
AU - Lin, Yu Cheng
PY - 2007
Y1 - 2007
N2 - This paper described the physicochemical characterization and application of quantum dots (CdSe/ZnS, QDs) whose surface were hydrophilic modified by mercaptoundecanoic acid (MUA). The stability kinetics of MUA-coated QDs (MUA-QDs) in aqueous solution was studied as a function of MUA-QDs concentration, type of light exposed, pH conditions and temperatures. The results show that the optimum concentration range of MUA-QDs is 0.5 to 1 mg/mL. We found that no obvious difference of fluorescence intensity was detected in MUA-QDs stored respectively at 4°C, 24°C and 44°C (8-hour). When they were exposed to UV light (312 nm) for 8 hr, their fluorescence intensity was shown serious decay. Moreover, the results showed that the pH of maximum stability of MUA-QDs in buffer solutions was 8. Finally, MUA-QDs had been endocytosis into H9c2 cells, a permanent cell line derived from rat cardiac tissue, for cell imaging application, the results provided the benefit to apply MUA-QDs in medical areas.
AB - This paper described the physicochemical characterization and application of quantum dots (CdSe/ZnS, QDs) whose surface were hydrophilic modified by mercaptoundecanoic acid (MUA). The stability kinetics of MUA-coated QDs (MUA-QDs) in aqueous solution was studied as a function of MUA-QDs concentration, type of light exposed, pH conditions and temperatures. The results show that the optimum concentration range of MUA-QDs is 0.5 to 1 mg/mL. We found that no obvious difference of fluorescence intensity was detected in MUA-QDs stored respectively at 4°C, 24°C and 44°C (8-hour). When they were exposed to UV light (312 nm) for 8 hr, their fluorescence intensity was shown serious decay. Moreover, the results showed that the pH of maximum stability of MUA-QDs in buffer solutions was 8. Finally, MUA-QDs had been endocytosis into H9c2 cells, a permanent cell line derived from rat cardiac tissue, for cell imaging application, the results provided the benefit to apply MUA-QDs in medical areas.
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U2 - 10.1117/12.708787
DO - 10.1117/12.708787
M3 - Conference contribution
AN - SCOPUS:34247381208
SN - 081946578X
SN - 9780819465788
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Microfluidics, BioMEMS, and Medical Microsystems V
Y2 - 22 January 2007 through 24 January 2007
ER -