Abstract
Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved.
Original language | English |
---|---|
Article number | A1768 |
Pages (from-to) | 1768-1777 |
Number of pages | 10 |
Journal | Biomedical Optics Express |
Volume | 5 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2014 Jun 1 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Atomic and Molecular Physics, and Optics