Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy

Chia Yuan Chang; Li Chung Cheng; Hung Wei Su; Yvonne Yuling Hu; Keng Chi Cho; Wei Chung Yen; Chris Xu; Chen Yuan Dong; Shean Jen Chen

doi:10.1364/BOE.5.001768

Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy

Chia Yuan Chang, Li Chung Cheng, Hung Wei Su, Yvonne Yuling Hu, Keng Chi Cho, Wei Chung Yen, Chris Xu, Chen Yuan Dong, Shean Jen Chen

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved.

Original language	English
Article number	A1768
Pages (from-to)	1768-1777
Number of pages	10
Journal	Biomedical Optics Express
Volume	5
Issue number	6
DOIs	https://doi.org/10.1364/BOE.5.001768
Publication status	Published - 2014 Jun 1

All Science Journal Classification (ASJC) codes

Biotechnology
Atomic and Molecular Physics, and Optics

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title = "Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy",

abstract = "Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved.",

author = "Chang, {Chia Yuan} and Cheng, {Li Chung} and Su, {Hung Wei} and Hu, {Yvonne Yuling} and Cho, {Keng Chi} and Yen, {Wei Chung} and Chris Xu and Dong, {Chen Yuan} and Chen, {Shean Jen}",

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doi = "10.1364/BOE.5.001768",

language = "English",

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Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy. / Chang, Chia Yuan; Cheng, Li Chung; Su, Hung Wei; Hu, Yvonne Yuling; Cho, Keng Chi; Yen, Wei Chung; Xu, Chris; Dong, Chen Yuan; Chen, Shean Jen.

In: Biomedical Optics Express, Vol. 5, No. 6, A1768, 01.06.2014, p. 1768-1777.

Research output: Contribution to journal › Article › peer-review

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AU - Chang, Chia Yuan

AU - Cheng, Li Chung

AU - Su, Hung Wei

AU - Hu, Yvonne Yuling

AU - Cho, Keng Chi

AU - Yen, Wei Chung

AU - Xu, Chris

AU - Dong, Chen Yuan

AU - Chen, Shean Jen

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AB - Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved.

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Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy

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