Wavelength-resolved flow cytometer under a dark-field illumination configuration

Shi Wei Lin, Pin Yao Wang, Angela Chen, Chih Han Chang, Che Hsin Lin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


This paper presents a novel yet high-performance diascopic illumination configuration for simultaneously detecting cells of different sizes and different fluorescence labeling in a microfluidic chip. An objective-type dark-field condenser equipped with a low-cost tungsten bulb light source with continuous wavelengths (400 to 900 nm) and an UV-Vis-NIR spectrometer is used for detecting the multispectral signals from various particles and cells. With this approach, continuous cell counting and spectra analyzing are realized in a single channel without using any spatial filter and delicate optical detectors. Results show that the developed system is capable of identifying different labeled particle and cell samples by extracting the information side-scatter, absorption, and fluorescence from the information-rich spectra. This proposed system provides an efficient multicolor detection for identification and classification of a microflow cytometer chip.

Original languageEnglish
Article number6030906
Pages (from-to)2845-2851
Number of pages7
JournalIEEE Sensors Journal
Issue number11
Publication statusPublished - 2011

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering


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