Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

Junjie Yao, Chih Hsien Huang, Lidai Wang, Joon Mo Yang, Liang Gao, Konstantin I. Maslov, Jun Zou, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning ORPAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

Original languageEnglish
Article number080505
JournalJournal of Biomedical Optics
Volume17
Issue number8
DOIs
Publication statusPublished - 2012 Aug

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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