TY - GEN
T1 - Widefield multiphoton microscopy with image-based adaptive optics
AU - Chang, Chia-Yuan
AU - Cheng, L. C.
AU - Su, H. W.
AU - Yen, W. C.
AU - Chen, S. J.
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Unlike conventional multiphoton microscopy according to pixel by pixel point scanning, a widefield multiphoton microscope based on spatiotemporal focusing has been developed to provide fast optical sectioning images at a frame rate over 100 Hz. In order to overcome the aberrations of the widefield multiphoton microscope and the wavefront distortion from turbid biospecimens, an image-based adaptive optics system (AOS) was integrated. The feedback control signal of the AOS was acquired according to locally maximize image intensity, which were provided by the widefield multiphoton excited microscope, by using a hill climbing algorithm. Then, the control signal was utilized to drive a deformable mirror in such a way as to eliminate the aberration and distortion. A R6G-doped PMMA thin film is also increased by 3.7-fold. Furthermore, the TPEF image quality of 1 μm fluorescent beads sealed in agarose gel at different depths is improved.
AB - Unlike conventional multiphoton microscopy according to pixel by pixel point scanning, a widefield multiphoton microscope based on spatiotemporal focusing has been developed to provide fast optical sectioning images at a frame rate over 100 Hz. In order to overcome the aberrations of the widefield multiphoton microscope and the wavefront distortion from turbid biospecimens, an image-based adaptive optics system (AOS) was integrated. The feedback control signal of the AOS was acquired according to locally maximize image intensity, which were provided by the widefield multiphoton excited microscope, by using a hill climbing algorithm. Then, the control signal was utilized to drive a deformable mirror in such a way as to eliminate the aberration and distortion. A R6G-doped PMMA thin film is also increased by 3.7-fold. Furthermore, the TPEF image quality of 1 μm fluorescent beads sealed in agarose gel at different depths is improved.
UR - https://www.scopus.com/pages/publications/84872918588
UR - https://www.scopus.com/pages/publications/84872918588#tab=citedBy
U2 - 10.1117/12.930327
DO - 10.1117/12.930327
M3 - Conference contribution
AN - SCOPUS:84872918588
SN - 9780819492371
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Unconventional Imaging and Wavefront Sensing 2012
T2 - Unconventional Imaging and Wavefront Sensing 2012
Y2 - 13 August 2012 through 14 August 2012
ER -