WWOX protein expression varies among RCC histotypes and downregulation of WWOX protein correlates with less-favorable prognosis in clear RCC

Jen Tai Lin, Tzong Shin Tzai, Chien Yu Liao, Jyh Seng Wang, Tony T. Wu, Hsiu Yu Wang, Chih Hsuan Wu, Chia Cheng Yu, Pei-Jung Lu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: WWOX has been shown to be a candidate tumor suppressor gene in numerous human cancers. The objective of this study is to determine the expression of WWOX in human renal cell carcinoma tumor cells and its possible correlation with clinical outcome. Methods: The WWOX protein expressions of human renal cell carcinoma (RCC) tumor and of matched normal renal parenchyma were examined, and its correlation with clinical cancer-specific survival was investigated. Results: Downregulation of WWOX only in clear cell type RCC was demonstrated in our results including immunohistochemistry, Western blot, and RT-PCR assay. For the remnant cell types of RCC, sample sizes were insufficient to draw any conclusion of WWOX protein expression. The decreased expression of WWOX in clear cell RCC tumor compared with matched normal renal parenchyma was also correlated with clinical cancer-specific survival (Kaplan-Meier, p = 0.0482). Conclusions: We proved that the expression level of WWOX is downregulated in human clear cell RCC. Moreover, the decreased expression of WWOX in clear cell RCC tumor compared with matched normal renal parenchyma was also correlated with clinical cancer-specific survival. Since clear cell RCC is a special human cancer using unique molecular pathogenesis, further investigation will provide more linking intracellular signaling of WWOX and novel therapeutic targets of human renal cell carcinoma.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalAnnals of Surgical Oncology
Volume20
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

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Renal Cell Carcinoma
Down-Regulation
Neoplasms
Proteins
Kidney
Survival
Tumor Suppressor Genes
Sample Size
Western Blotting
Immunohistochemistry
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

Lin, Jen Tai ; Tzai, Tzong Shin ; Liao, Chien Yu ; Wang, Jyh Seng ; Wu, Tony T. ; Wang, Hsiu Yu ; Wu, Chih Hsuan ; Yu, Chia Cheng ; Lu, Pei-Jung. / WWOX protein expression varies among RCC histotypes and downregulation of WWOX protein correlates with less-favorable prognosis in clear RCC. In: Annals of Surgical Oncology. 2013 ; Vol. 20, No. 1. pp. 193-199.
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abstract = "Background: WWOX has been shown to be a candidate tumor suppressor gene in numerous human cancers. The objective of this study is to determine the expression of WWOX in human renal cell carcinoma tumor cells and its possible correlation with clinical outcome. Methods: The WWOX protein expressions of human renal cell carcinoma (RCC) tumor and of matched normal renal parenchyma were examined, and its correlation with clinical cancer-specific survival was investigated. Results: Downregulation of WWOX only in clear cell type RCC was demonstrated in our results including immunohistochemistry, Western blot, and RT-PCR assay. For the remnant cell types of RCC, sample sizes were insufficient to draw any conclusion of WWOX protein expression. The decreased expression of WWOX in clear cell RCC tumor compared with matched normal renal parenchyma was also correlated with clinical cancer-specific survival (Kaplan-Meier, p = 0.0482). Conclusions: We proved that the expression level of WWOX is downregulated in human clear cell RCC. Moreover, the decreased expression of WWOX in clear cell RCC tumor compared with matched normal renal parenchyma was also correlated with clinical cancer-specific survival. Since clear cell RCC is a special human cancer using unique molecular pathogenesis, further investigation will provide more linking intracellular signaling of WWOX and novel therapeutic targets of human renal cell carcinoma.",
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WWOX protein expression varies among RCC histotypes and downregulation of WWOX protein correlates with less-favorable prognosis in clear RCC. / Lin, Jen Tai; Tzai, Tzong Shin; Liao, Chien Yu; Wang, Jyh Seng; Wu, Tony T.; Wang, Hsiu Yu; Wu, Chih Hsuan; Yu, Chia Cheng; Lu, Pei-Jung.

In: Annals of Surgical Oncology, Vol. 20, No. 1, 01.01.2013, p. 193-199.

Research output: Contribution to journalArticle

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T1 - WWOX protein expression varies among RCC histotypes and downregulation of WWOX protein correlates with less-favorable prognosis in clear RCC

AU - Lin, Jen Tai

AU - Tzai, Tzong Shin

AU - Liao, Chien Yu

AU - Wang, Jyh Seng

AU - Wu, Tony T.

AU - Wang, Hsiu Yu

AU - Wu, Chih Hsuan

AU - Yu, Chia Cheng

AU - Lu, Pei-Jung

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Background: WWOX has been shown to be a candidate tumor suppressor gene in numerous human cancers. The objective of this study is to determine the expression of WWOX in human renal cell carcinoma tumor cells and its possible correlation with clinical outcome. Methods: The WWOX protein expressions of human renal cell carcinoma (RCC) tumor and of matched normal renal parenchyma were examined, and its correlation with clinical cancer-specific survival was investigated. Results: Downregulation of WWOX only in clear cell type RCC was demonstrated in our results including immunohistochemistry, Western blot, and RT-PCR assay. For the remnant cell types of RCC, sample sizes were insufficient to draw any conclusion of WWOX protein expression. The decreased expression of WWOX in clear cell RCC tumor compared with matched normal renal parenchyma was also correlated with clinical cancer-specific survival (Kaplan-Meier, p = 0.0482). Conclusions: We proved that the expression level of WWOX is downregulated in human clear cell RCC. Moreover, the decreased expression of WWOX in clear cell RCC tumor compared with matched normal renal parenchyma was also correlated with clinical cancer-specific survival. Since clear cell RCC is a special human cancer using unique molecular pathogenesis, further investigation will provide more linking intracellular signaling of WWOX and novel therapeutic targets of human renal cell carcinoma.

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