Zap70 controls the interaction of talin with integrin to regulate the chemotactic directionality of T-cell migration

Yu Ping Lin, Yu Jung Cheng, Jyun Yuan Huang, Huan Ching Lin, Bei Chang Yang

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Aberrant lymphocyte infiltration is crucial for many disorders such as tumor immune escape and autoimmunity. In this study, we have investigated T-cell migration in a three-dimensional collagen matrix containing tumor spheroids and by using μ-Slide chemotaxis and found that Zap70 regulates directionality during cell chemotaxis. Jurkat cells actively migrated toward SDF-1, nutrition, and spheroids of MCF-7 breast carcinoma cells embedded in collagen matrix. Inhibition of Zap70 activity impaired transmigration and μ-Slide chemotaxis but not the random movement of T cells in the collagen/fibronectin matrix. P116 cells, a Zap70 deficient variant of Jurkat, showed active random movement but failed to migrate against chemoattractants. P116 cells exhibited a reduced polarization of cell morphology, showing less lamellipodia formation accompanied with a fast pseudopod turnover rate. Instead of direct interacting with F-actin, Zap70 formed a complex with talin which is an integrin scaffold for F-actin. SDF-1 enhanced Zap70 phosphorylation and also stimulated binding of talin and β1 integrin activation. P116 cells showed reduced complex of talin and β1 integrin in parallel with impaired integrin activation. Collectively, Zap70 modulates integrin activation by interacting with talin, which contributes to directionality of T-cell migration, severing as a potential target for anti-inflammation therapy.

Original languageEnglish
Pages (from-to)2022-2029
Number of pages8
JournalMolecular Immunology
Volume47
Issue number11-12
DOIs
Publication statusPublished - 2010 Jul

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

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