TY - GEN
T1 - Zebrafish sperm activation through an artificial cilia embedded serpentine microfluidic platform
AU - Panigrahi, Bivas
AU - Lu, Chang Hung
AU - Ghayal, Neha
AU - Chen, Chia Yuan
N1 - Funding Information:
This study was supported through Ministry of Science and Technology of Taiwan under Contract No. MOST 105-2628-E-006-006-MY3 (to Chia-Yuan Chen). This work would not be possible without the facility provided by Center for Micro/Nano Science and Technology, National Cheng Kung University. The research was in part supported by the Minsitry of Education, Taiwan, R. O. C. through the Aim for the Top University Project to National Cheng Kung University (NCKU).
Publisher Copyright:
© 2018 IEEE.
PY - 2018/4/24
Y1 - 2018/4/24
N2 - This work reports an artificial cilia embedded serpentine microfluidic channel for high throughput zebrafish sperm cell activation. With this proposed device, an average of 74.44 ± 6.07 % of the used zebrafish sperm cells was activated within the first 15 seconds of the experiment. which is at least 20 % superior to the currently other available techniques. The μPIV analysis of the flow zone suggests that the curvilinear microchannel initiates an orbital rotation to the flow field whereas artificial cilia motion establishes a local rotation in the center of the flow field. Collectively. both the rotational motion. harness a uniform mixing within the microchannel which is quintessential towards changing the temporal dynamics of the sperm cells' hence promoting the sperm cell activation.
AB - This work reports an artificial cilia embedded serpentine microfluidic channel for high throughput zebrafish sperm cell activation. With this proposed device, an average of 74.44 ± 6.07 % of the used zebrafish sperm cells was activated within the first 15 seconds of the experiment. which is at least 20 % superior to the currently other available techniques. The μPIV analysis of the flow zone suggests that the curvilinear microchannel initiates an orbital rotation to the flow field whereas artificial cilia motion establishes a local rotation in the center of the flow field. Collectively. both the rotational motion. harness a uniform mixing within the microchannel which is quintessential towards changing the temporal dynamics of the sperm cells' hence promoting the sperm cell activation.
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U2 - 10.1109/MEMSYS.2018.8346468
DO - 10.1109/MEMSYS.2018.8346468
M3 - Conference contribution
AN - SCOPUS:85047010594
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 10
EP - 13
BT - 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
Y2 - 21 January 2018 through 25 January 2018
ER -