TY - JOUR
T1 - Zinc induces mitogen-activated protein kinase activation mediated by reactive oxygen species in rice roots
AU - Lin, Chung Wen
AU - Chang, Hsien Bing
AU - Huang, Hao Jen
PY - 2005/10/1
Y1 - 2005/10/1
N2 - It is well known that zinc (Zn) is one of the micronutrients essential for normal growth and development of plants. However, the molecular mechanisms responsible for the regulation of plant growth by Zn are still not completely understood. The aim of this study was to investigate the signalling transduction pathways activated by Zn. We show that Zn elicited a remarkable increase in myelin basic protein (MBP) kinase activities. By immunoblot analysis, we suggest that Zn-activated 40- and 42-kDa MBP kinases are mitogen-activated protein kinases (MAPK). Pre-treatment of rice roots with reactive oxygen species (ROS) scavenger, sodium benzoate, was able to effectively prevent Zn-induced MAPK activation. However, phosphoinositide 3-kinase (PI-3K) inhibitor, LY294002, was unable to inhibit Zn-induced MAPK activation. These results suggest that the ROS may function in the Zn-triggered MAPK signalling pathway in rice roots.
AB - It is well known that zinc (Zn) is one of the micronutrients essential for normal growth and development of plants. However, the molecular mechanisms responsible for the regulation of plant growth by Zn are still not completely understood. The aim of this study was to investigate the signalling transduction pathways activated by Zn. We show that Zn elicited a remarkable increase in myelin basic protein (MBP) kinase activities. By immunoblot analysis, we suggest that Zn-activated 40- and 42-kDa MBP kinases are mitogen-activated protein kinases (MAPK). Pre-treatment of rice roots with reactive oxygen species (ROS) scavenger, sodium benzoate, was able to effectively prevent Zn-induced MAPK activation. However, phosphoinositide 3-kinase (PI-3K) inhibitor, LY294002, was unable to inhibit Zn-induced MAPK activation. These results suggest that the ROS may function in the Zn-triggered MAPK signalling pathway in rice roots.
UR - http://www.scopus.com/inward/record.url?scp=28944451572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=28944451572&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2005.10.001
DO - 10.1016/j.plaphy.2005.10.001
M3 - Article
C2 - 16324848
AN - SCOPUS:28944451572
VL - 43
SP - 963
EP - 968
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
SN - 0981-9428
IS - 10-11
ER -