3D Dielectrophoretic microfluidic chip for rapid clinical diagnosis of dengue infection

Abstract

Dengue is an emerging viral infection that is increasing annually worldwide According to World Health Organization (WHO) when the dengue viruses are transferred by the mosquito-borne to humans the virus can be detected in serum and plasma circulating blood cells and other tissues for 4 - 5 days after the onset of febrile illness Hence a rapid sensitive and accurate result of the dengue infection diagnosis is a crucial tool to provide proper treatment prevention and management to the disease In this study the diagnosis of dengue virus (DENV) infection based on microfluidic dielectrophoresis (DEP) chip was introduced The method used in this study was hydrodynamic and DEP forces to allow the immuno-reaction between 4G2-coated beads and fluorescence-labeled DENV particles on the designed chip The DEP force was employed to capture the modified beads (anti-flavivirus monoclonal antibody-coated beads) flowing in the microfluidic chip and the DENV modified with fluorescence label could be captured on the modified beads by immunoreaction The obtained fluorescent signal was then quantified by ImageJ analysis software A small amount of sample (10 - 15 μL) was needed for DENV detection on the DEP chip The platform can be used to accelerate an immuno-reaction time in which the on-chip detection time was less than 3 min demonstrating an ability for DENV detection as low as 102 pfu/mL using the clinical dengue virus (DENV) sample

Date of Award	2019
Original language	English
Supervisor	Hsien-Chang Chang (Supervisor)