A Cell Viability Study of Non-Thermal Micro-Plasma Exposure on Malignant Melanoma Cells

  • 吳 彥緯

Student thesis: Master's Thesis


Recent researches have proven that non-thermal micro-plasma (NTP) can inactivate cancer cells with low side effects to the human body NTP is a gas that ionized partially which contain energy particles including neutral atoms electrons and ions Previously NTP was directly applied to cells or tumors but the injured area and depth is limited; hence a new indirect plasma exposure method was investigated which known as Plasma Activated Medium (PAM) The PAM method means only medium was exposed to NTP any direct exposure from NTP to target cells was excluded; the exposed medium was then provided to target cells or injected into tumors PAM was confirmed having the same anti-cancer effect as direct plasma exposure most importantly PAM can ignore the exposure depth limit and cover a larger injured area that direct plasma exposure can’t achieve However PAM was less effective than direct plasma exposure but how exactly lower was remain unknown in this study the ability of PAM will be affirmed by using direct plasma exposure as a reference in contrast with PAM Malignant melanoma cells B16-F10 A375 and MeWo cells the most dangerous type of skin cancer cells was used in this study the reason for choosing skin cancer as target cells are due to malignant melanoma is easily reachable by either direct plasma exposure or PAM The result shows that the effect of PAM was only briefly lower than direct plasma exposure Furthermore NTP has a great effect on B16-F10 and MeWo cells by both direct exposure and PAM On the other hand A375 cells show strong resistance to NTP PAM is a promoting methodology it has the potential to become a new option for skin cancer cure in the future
Date of Award2018 Aug 2
Original languageEnglish
SupervisorJiunn-Der Liao (Supervisor)

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