A New Microfluidic Device for Mercury (II) Ion Detection Based on Graphene Oxide for Aptasensor in FRET

Abstract

Heavy metals emitted into the environment through human activities causes environmental pollution and threats to living things Once the heavy metal is released whether it is eroded into the sea or accumulated in the aquifer it may interact with the organic matter between the organism Therefore many human diseases for example cardiovascular blood lung digestive system kidney system immune system nervous system endocrine system reproductive system and fetus may be due to suffering from the toxic heavy metals in our environment In this study we design a microfluidic device to detect the concentration of Hg2+ in liquids The microchannel is fabricated by using Polydimethylsiloxane which can accelerate the reaction and enhance the mixing efficiency We use Aptamer together with graphene oxide mixed inside the microchannels when in the presence of Hg2+ thymine selectively combined with Hg2+ to form T–Hg2+–T complex This could get rid of the adsorption of Aptamer by graphene oxide and thus can recovered the fluorescence This study indicates the detection range is from 2 nM to 100 nM in microchannel (R2=0 98114) and according to the International World Health Organization (WHO) the maximum allowable mercury ion is 6 ppb in drinking water and the minimum detection of limit (LOD) is 2 nM which is equivalent to 0 39 ppb From the result the detection of limit is obvious lower than the regulations

Date of Award	2019
Original language	English
Supervisor	Ruey-Jen Yang (Supervisor)