A study of the relationship between ovalbumin allergen exposure and adipose tissue inflammation insulin resistance and cardiac damage

  • 榮 建誠

Student thesis: Doctoral Thesis

Abstract

Allergens such as pollen dust mites and fungi are a type of antigen that induces immune reactions Many studies have demonstrated that exposure to allergens can increase the risk of asthma allergic rhinitis and dermatitis Recent studies also have indicated that the pollen level is increasing and that warm weather supports appropriate conditions for the growth of dust mites and fungi Therefore we cannot neglect the health effects of human allergen exposure Recently some studies have shown that exposure to allergens might be one risk factor related to cardiovascular diseases but the mechanisms are still unknown According to previous studies compared to a healthy population patients with allergic and cardiovascular diseases have consistent trends in regard to the levels of adipokines from adipose tissues which play a key role in cardiovascular diseases and patients with allergic diseases have a higher risk of cardiovascular diseases Moreover a few animal studies have indicated that exposure to allergens can reduce the level of adiponectin which plays a protective role in cardiovascular diseases Therefore adipose tissues may play an important role in the mechanisms connecting allergen exposure and cardiovascular diseases However it is still not understood why exposure to allergens can reduce the level of adiponectin and no study has yet investigated whether exposure to allergens further involves the pathogenesis of cardiovascular diseases Moreover adipokines from adipose tissues are associated with insulin resistance and no study has to this date investigated the relationship between allergen exposure and insulin resistance Finally Th cells play important roles in involving the inflammation of airway and adipose tissues but we are not still understand whether Th cells of airway affect the inflammation situation in adipose tissues In this study there were three objectives: (1) to collect bronchoalveolar lavage fluid (BALF) (total cell specific cell and IL-5) blood (total IgE OVA-specific IgE adiponectin IL-6 and TNF-?) and adipose tissue (IL-6 TNF-? and HE statin) samples using the acute OVA allergen exposure model (control mice with PBS) and to investigate why exposure to OVA allergens can reduce the level of adiponectin in the blood (2) to collect blood (total IgE resistin adiponectin IL-6 TNF-? VCAM-1 ICAM-1 glucose insulin LDH CPK and CK-MB) and adipose tissue (IL-6 TNF-? PAI-1 IL-1β Mgl1 Mgl2 IL-10 and HE and IHC statin) samples using the chronic exposure model (control mice with PBS) in order to study whether exposure to OVA allergens can affect the expression of biomarkers in subjects with cardiovascular diseases and insulin resistance as well as further involve the pathogenesis of insulin resistance and cardiovascular diseases and (3) to investigate how OVA allergens affect the characteristics of adipose tissues by conducting a comparison between acute- and chronic-exposure models Our data showed that exposure to OVA allergens not only induced airway inflammation but also affected the characteristics of adipose tissues and further reduced the expressions of adiponectin in serum and adipose tissues according to the acute-exposure model The chronic-exposure model showed that there were higher expressions of pro-inflammation IL-6 TNF-? and IL-1β and lower levels of anti-inflammation IL-10 and Mgl2 in mice that were OVA-challenge compared with mice that were PBS-challenged and the weight of the adipose tissue was lower in OVA-challenged mice In addition HE and IHC stains also indicated that there were significant expressions of macrophage and F4/80 respectively Furthermore mice that were OVA-challenged had higher serum levels of IL-6 E-selectin and resistin and their adiponectin levels were lower when compared to those that were PBS challenged Serum E-selectin resistin and adiponectin were significantly correlated with pro-inflammation indexes in adipose tissues Our data also revealed that there were higher levels of HOMA-IR and inflammatory mediators with circulatory system in mice that were OVA-challenged as compared to those that were PBS-challenged and HOMA-IR and inflammatory mediators with circulatory system were both associated with resistin respectively These results demonstrated that exposure to OVA allergens can induce inflammation of adipose tissues and affect the levels of biomarkers in subjects with inflammation of circulatory system and insulin resistance in serum Finally this study indicated that OVA allergen exposure causes imbalances between Th1 type and Th2 type cells in adipose tissues which further affects the expressions of M1 and M2 in such tissues However our data cannot demonstrate whether Th cells of airway affect the inflammation reactions of adipose tissues This study not only explains the possible mechanisms between pollen and cardiovascular system but also demonstrates that adipose tissues play an important role in the relationship between allergen exposure and insulin resistance The results of this study support directions for disease prevention and treatment
Date of Award2014 Nov 27
Original languageEnglish
SupervisorHuey-Jen Su (Supervisor)

Cite this

A study of the relationship between ovalbumin allergen exposure and adipose tissue inflammation insulin resistance and cardiac damage
建誠, 榮. (Author). 2014 Nov 27

Student thesis: Doctoral Thesis