Application of Galectin-1 aptamers in fibroblast activity

  • 楊 東錦

Student thesis: Master's Thesis

Abstract

Aptamers are short DNA or RNA sequence that can bind to specific sites on proteins through its secondary conformation and structure The binding of the aptamer to the protein can result in blockage of specific protein functions Previous studies from our group have identified aptamers AP-27 AP-39 and AP-74 which possess high binding affinity for galectin-1 (Gal-1) a member of galectin family that has a carbohydrate recognition domain (CRD) to bind to β-galactosides Gal-1 plays a critical role in the regulation of cell adhesion migration tumorigenesis and promoting cancer cells metastasis Furthermore we also found that Gal-1 influences the several biological functions of normal cells such as promoting fibroblast proliferation and activation Activation of fibroblasts plays important role in several physiological and pathological processes such as wound healing and liver fibrosis We have demonstrated that AP-27 and AP-39 dose-dependently inhibit Gal-1-induced oral cancer cell adhesion In this study we found that the aptamers efficiently rescued Gal-1-activated fibroblast migration and proliferation These aptamers especially AP-74 decreased Gal-1-induced phosphorylation of Smad3 in fibroblasts On the other hand we also found that these aptamers inhibited Gal-1-induced lung cancer cell migration and might through inhibiting Akt/mTOR signaling pathway In the future the Gal-1 aptamers will be optimized by screening a custom aptamer array chip to search out the effective region of Gal-1 aptamers for costing down and further applications The development of Gal-1 aptamer-based therapeutics may provide unique clinical opportunities for diseases associated with fibroblast activation and preventing cancer cell metastasis
Date of Award2014 Aug 28
Original languageEnglish
SupervisorYuh-Ling Chen (Supervisor)

Cite this

Application of Galectin-1 aptamers in fibroblast activity
東錦, 楊. (Author). 2014 Aug 28

Student thesis: Master's Thesis