Biological effects of Schumann Resonance Frequency modulation on B16-F10 melanoma skin cancer cells

  • 唐 敬堯

Student thesis: Doctoral Thesis


Melanoma is the most dangerous type of skin cancer because it is a particularly aggressive form of tumor Patients with invasive melanoma have a poor prognosis following surgery or treatment with chemotherapies and hence the use of an extremely low frequency electromagnetic field (ELF-EMF) is an alternative treatment method that could alter melanoma invasion and growth Therefore researchers are increasingly investigating the inhibitory effects of different EMF frequency types on B16-F10 cancer cells In this study we used the Schumann resonance frequency (7 83 Hz) on cell with melanoma combined with ELF-EMF exposure for 48 hr to influence B16-F10 cancer cells Additionally we used different sweep frequency ranges (step intervals 0 1 and 0 05 Hz) around 7 83 Hz to explore the different biological responses We detected the viability of cancer cells via 3-(4 5-dimethythiazol-2-y1)-2 5-dipheny1 tetrazolium bromide (MTT assay) and used the calcium fluorescence dye Fluo-4 AM to show intracellular calcium fluorescence which is positively proportionate to intracellular calcium concentration The Schumann sweep frequency (7 83 ± 0 3 Hz) had the highest inhibitory rate (25 5%) on cell viability Furthermore we used the zoom fast Fourier transform method to analyze the sweep frequency spectrum’s magnetic field exposure in terms of cell duration time The cell inhibition rate was 21 4–25 45% when B16-F10 cells were exposed to a 7 83-Hz EMF for more than 2 3 hr Moreover the cell inhibition rate decreased 6% and 12% for sweep frequencies at ± 1 Hz and ± 2 Hz respectively Thus the experimental results showed an obviously inhibitory effect around 7 83 Hz frequency spectrum with different frequency sweep intervals
Date of Award2019
Original languageEnglish
SupervisorLing-Sheng Jang (Supervisor)

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