Cell Proliferation Adhesion and Migration on the Nanopillar Srray Structure with Anodic Aluminum Oxide Process

Abstract

Within the extracellular matrix the naturally occurring nanotopographic structures surrounding cells have been proved to have influence on cell morphology alignment adhesion migration proliferation and cytoskeleton organization In a particular case the influence of micro and nano topographic on cells may even replace chemical stimulation The clearer recognition of environmental effect at cellular level will create many potential applications and assist human in providing the foundations for new avenues of biomedical research in the near future Therefore various studies developed alternative patterns to determine the important signaling modality in controlling cell function by investigating the interaction of mammalian cells with mechanotransductive cues In this study Anodic Aluminum Oxide (AAO) was served as template to replicate UV curable membrane with high density nanopillar array including the diameter of 80 nm and 200 nm Adipose-derived stem cells (ADSCs) seeded on the nanostructure substrate membranes showed the biocompatibility of UV gel and the effect of physical cues on cell proliferation by WST-1 cell proliferation assay That is compared to a flat control nanopatterned substrates not increased the growth rate of cells Furthermore SEM images displayed cell attachment and filopodia extension without interfering by the gap between nanopillars Time lapse observation and wound healing assay respectively recorded ADSCs and MG63 cells (human osteosarcoma cell line) migrated at a faster speed on the nanopillar surface Immunofluorescence showed ADSCs and MG63 have obvious focal adhesion and stress fiber in the control group and have better adhesion and bigger spread area than other groups By numbers compared to the speed on the flat control (0 56 ?m/min) ADSCs with fastest migration (1 04 ?m/min) on the high density nanopillars membrane of 80 nm diameter were markedly higher than the nanopillars membrane of 200 nm diameter (0 82 ?m/min) It is noteworthy that MG63 migrated and spread alone on the nanopillars array which is opposed to the performance of MG63 on the flat substrate that spread slowly with gradual proliferation

Date of Award	2016 Sept 5
Original language	English
Supervisor	Ming-Long Yeh (Supervisor)