Characterization of RAG and TCR-? genes at early stage in giant grouper

Abstract

During the early stages of T lymphocytes and B lymphocytes the diversity of functional T cell receptors and immunoglobulins is accomplished through V(D)J recombination and is regulated by the recombination activating genes RAG1 and RAG2 Therefore this research characterizes the recombination activating genes and T lymphocytes at an early stage in giant grouper The results of real-time PCR show that RAG1 RAG2 and TCR-? exhibit high expression at the kitting stage which is about 12 to 14 days and the diskitting stage at about 30 days which is indicated that the period of morphology changes is also an important period for the development of adaptive immunity in early stage of giant grouper To investigate the diversity of T cell receptors in giant grouper multiple sequence alignment is performed on the V?-J? junction of ggTCR-? The results show that the sequences of V?-J? junction are variable which can result in ggTCR-? diversity allows recognition of different antigens In addition the results of a qPCR-based V(D)J recombination assay and DNA-fiber fluorescence in situ hybridization show that the V(D)J recombination event is highly expressed in the thymus which is indicated that thymus is an important organ for T cell development These results provide a reference time point for the development of adaptive immunity in giant grouper verified the V(D)J recombination event of TCR-? in giant grouper and demonstrated that the thymus could be an important organ for the development of T lymphocytes It is expected to contribute to the research on the resistance of early stage giant grouper to foreign pathogens and efforts to reduce losses in the aquaculture industry from diseases

Date of Award	2021
Original language	English
Supervisor	Tzong-Yueh Chen (Supervisor)