Chromosome mapping of genetic markers on chromosome 7 Phalaenopsis aphrodite subsp formosana

  • 許 惠嵐

Student thesis: Master's Thesis

Abstract

Orchidaceae is the largest flowering family which consists of at least 25 000 species Phalaenopsis an Orchidaceae genus is a high-value ornamental plant in global horticulture market Studying Phalaenopsis genomes will benefit our knowledge of orchid biology Up to now there is only one species that its draft genome sequence has been determined in Phalaenopsis Phalaenopsis aphrodite subsp formosana is an important parental material in breeding and one of model organisms for studying orchid biology Due to different assembly strategies they resulted in inconsistent linkage groups Cytogenetic maps can ensure the accuracy of linkage groups but small-size metaphase chromosomes of P aphrodite limit cytogenetic researches and applications In this study we developed a modified drop method for pachytene chromosomes preparation in P aphrodite and examined its applicability for fluorescent in situ hybridization (FISH) technique By mapping four scaffolds of D_L10 linkage group to high-resolution pachytene chromosomes we validated the genome assembly of P aphrodite and found that there are differences of recombination frequency among different chromosome regions Also the chromosome which D_L10 linkage group located on was identified as chromosome 7 of P aphrodite Additionally we examined that pachytene chromosomes of P aphrodite can apply on chromosome laser capture microdissection (LCM) which could establish chromosome-specific library The development of modified drop method for pachytene chromosome preparation would accelerate the integration of genetic map and chromosome map and also benefit the genomic and cytogenetic researches in P aphrodite
Date of Award2016 May 23
Original languageEnglish
SupervisorSong-Bin Chang (Supervisor)

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