Comparative FISH mapping of 4 linkage groups between Phalaenopsis aphrodite and Phalaenopsis equestris

Abstract

With more than 28000 species Orchidaceae is the biggest family in angiosperms Phalaenopsis becomes one of the most popular groups of orchids because of their various morphology and colors In Taiwan there are two native Phalaenopsis species P aphrodite and P equestris Both of them are served as important parents in orchid breeding programs Therefore understanding and revealing the genetic information of the two species by cytogenetic analysis will be beneficial to orchid breeding Here we selected four linkage groups of P aphrodite and systematically identify their positions on pachytene chromosomes of P equestris by comparative fluorescence in situ hybridization (FISH) mapping Our results showed that the four linkage groups are conserved and there is collinearity between some chromosomes of P aphrodite and their corresponding chromosomes in P equestris We also identified chromosomal rearrangements between the two species Overall our results revealed the precise positions of the four linkage groups on a single chromosome in P equestris which will facilitate the identification of each chromosome The results gained in this study will contribute to systematic orchid breeding and enhance the understanding of orchid evolution

Date of Award	2019
Original language	English
Supervisor	Song-Bin Chang (Supervisor)