Comparison of the running exercise-induced neural activity in different brain regions

  • 劉 玉雯

Student thesis: Master's Thesis

Abstract

Background: Exercise benefits brain functions such as increases neuronal activity and synaptic plasticity which has been linked to brain-derived neurotrophic factor (BDNF) c-fos an immediate early gene is frequently used as neuron activation marker Exercise increases the expression of c-Fos in some brain regions such as hippocampus However it is unclear whether the effect of exercise on brain is ubiquitous or is a region-specific phenomenon Objective: To identify the brain regions sensitive to running Methods: C57BL/6J mice 8-week-old were forced to run on a treadmill at moderate intensity one hour each time The mice were assigned to the single-bout (acute) or one month (long-term) exercise group The mice were either killed immediately (acute: E1h; long-term: 1M-E1h) or 2 h after (acute: E1hS2h; long-term: 1M-E1hS2h) the 1 h running Mice of the acute control group (Ctrl) were killed without running Mice of the long-term control group (1M-Ctrl) omitted the last run and killed at the same time as the 1M-E1h group The brains were cut at 25 μm and immunostained for c-Fos The number of c-Fos+ cells was counted in the following brain regions: cortex (motor cortex somatosensory cortex) limbic area (cingulate piriform cortex septal nucleus hippocampus amygdala) basal nuclei (caudate/putamen accumbens nucleus core) diencephalon (thalamus hypothalamus) brain stem (substantia nigra ventral tegmental area superior colliculus lateral periaqueductal gray dorsal raphe nucleus pontine nucleus) and cerebellum (anterior and posterior lobes) Some of the brains were dissect into primary motor cortex primary somatosensory cortex dorsal and ventral parts of hippocampus amygdala caudate/putamen thalamus and hypothalamus to measure the levels of BDNF Results: Compared to the Ctrl group the density of c-Fos+ cells was increased in the cortex hippocampus and basal nuclei of E1h group In the E1hS2h group a few regions of hippocampus were still higher than those of the Ctrl group In the long-term exercise mice the density of c-Fos+ cells of cortex limbic area basal nuclei diencephalon and lateral periaqueductal gray of brain stem in the 1M-E1h group were higher than those of 1M-Ctrl group The density of c-Fos+ cells in the regions of hippocampus piriform cortex caudate/putamen hypothalamus and lateral periaqueductal gray remained elevated in the 1M-E1hS2h group Although the levels of BDNF were not affected by acute exercise the changed levels of BDNF were positively correlated with the changed density of c-Fos+ cells Conclusion: Both in acute and long-term exercises influence neuronal activity in the cortex hippocampus and basal nuclei while neuronal activity in the diencephalon and brain stem were only influenced by long-term exercise Acute exercise-induced changes in the BDNF levels were positively correlated with changes in the density of c-Fos+ cells Physical exercise may serve as a non-pharmaceutical means to protect brain especially those regions responding to exercise training
Date of Award2016 Jan 28
Original languageEnglish
SupervisorYu-Min Kuo (Supervisor)

Cite this

Comparison of the running exercise-induced neural activity in different brain regions
玉雯, 劉. (Author). 2016 Jan 28

Student thesis: Master's Thesis