Control and Growth Inhibition of Microcystis using Beta-cyclocitral

  • 葉 桂寶

Student thesis: Master's Thesis


Microcystis aeruginosa has been known to produce beta-cyclocitral when the cells ruptured The chemical was known to cause rupture of cyanobacteria and algae In this study effect of beta-cyclocitral on the cell integrity and growth inhibition of two cyanobacteria Microcystis aeruginosa and Cylindrospermopsis raciborskii were studied A flowcytometer coupled with SYTOX stain was used to quantify the cell integrity of the tested cyanobacteria while an enzyme immunosorbent assay was used to determine the cyanotoxins produced by the two cyanobacteria Kinetic experiments show that 1 mg L-1 of beta-cyclocitral was able to cause 50% and 15% cell rupture of the tested Microcystis and Cylindrospermopsis respectively A first-order rate model was able to describe the data of cell integrity for the first 60 minutes of rupture Four mechanical methods including sonication bead mill freeze method and sonication enhanced with bead mill were tested for the production of beta-cyclocitral from Microcystis cells The results show that 5 freezing cycles of the freeze method is able to produce beta cyclocitral up to 15 ?g L-1 with 92% efficiency and 84 3% recovery respectively Furthermore beta-cyclocitral was produced from sonication of Microcystis cell and then applied for inhibition of M aeruginosa and C raciborskii The experimental results may provide information of production and application of the cyanobactericide beta-cyclocitral on the control of cyanobacteria in water bodies
Date of Award2018 Feb 13
Original languageEnglish
SupervisorTsair-Fuh Lin (Supervisor)

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