Establishment of chromosome-specific markers in Erycina pusilla

Abstract

Erycina pusilla is a good plant model in orchid species since it has several advantages such as a tiny adult plant shorter generation time than other wild and commercialized orchids all year blooming a relatively small genome size and low chromosome number Although the conventional karyotype analysis in E pusilla has been reported neither the chromosome pattern nor the arm ratio can be used for precise chromosome identification Therefore it needs specific markers to help distinguishing chromosomes In this study we selected gene fragments of EpPEPC EpEFS EpCMT EpERS EpMDAR and EpSOC1 as tyramide-FISH probes using the full-length corresponding gene sequences of Phalaenopsis equestris as references Tyramide-FISH can be used to detect smaller probes (less than 1 kb) than conventional FISH In tyramide-FISH analysis signals of EpPEPC were located on the terminal sites of every pair of chromosomes The signals of EpERS and EpCMT which were located on all same chromosomes with the signals of 5S rDNA were respectively located on the short arm of the first pair of chromosomes and the long arm of the fifth pair of chromosomes The signals of EpEFS were located on the short arm of the third pair of chromosomes and the signals of EpMDAR were located on the short arm of the second pair of chromosomes With these discriminative signals on chromosomes we have established the specific markers for chromosome identification in E pusilla

Date of Award	2018 Aug 1
Original language	English
Supervisor	Song-Bin Chang (Supervisor)