Evaluation of tetracycline loaded calcium deficient hydroxyapatite for integrated anti-microbial activity

  • 謝 秉舟

Student thesis: Master's Thesis


Peri-implantits is a major risk factor for late dental implants failures Traditional remedy usually failed to eliminate pathogens completely due to biofilm covering the titanium surface In this study we developed a nano calcium-deficient hydroxyapatite (CDHA) carrier intended for extended tetracycline (TC) release to disrupt biofilm on the implant surface while simultaneously enhance new bone regeneration for peri-implantitis treatment The tetracycline-loaded CDHA nanoparticles (TC-CDHA) were characterized by scanning electron microscope (SEM) transmission electron microscope (TEM) X-ray diffraction (XRD) energy-dispersive X-ray spectroscopy (EDS) and dynamic light scattering (DLS) The TC-CDHAs were then evaluated for anti-bacteria potency by determine their minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of pathogens The TC-CDHA presented as round less than 100nm rod shape particles under SEM and TEM The crystalline size measured by Scherrer’s formula is about 10 8nm x 4 62nm We discovered that the loading capacity of TC was 86 23% Tetracycline is well embedded in the CDHA as revealed by EDS examination Anti-bacterial assay showed that the MIC of the drug loaded nanoparticles are comparable to that of the freeform TC (86 25% for TC vs 89 47% for TC-CDHA) when both were at TC concentration of 0 1ug/ml This study revealed that the as prepared TC-CDHA has similar potency as freeform TC while may harbor additional advantages for stimulating bone regeneration Such nanomedicine holds a promising future for peri-implantitis treatment
Date of Award2018 Feb 7
Original languageEnglish
SupervisorJehn-Shun Huang (Supervisor)

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