Fabrication of Hydrophilic Polymer Beads Using as Scaffolds for Cell Culture

Abstract

Polymer beads have gained a lot of interest in tissue engineering due to the construction of three dimensional (3-D) porous scaffolds for implanting into a defect site Polymer beads which are spherical particles offer high cell yields in small volumes due to high surface area to volume ratio of polymeric particles In this research preparations of poly(2-hydroxyethyl methacrylate) (poly(HEMA)) in microparticle and nanoparticle size were successfully synthesized via suspension and emulsion polymerization techniques In microparticle a positively charged monomer of 2-(diethylamino)ethyl methacrylate (DEAEMA) was randomly copolymerized with HEMA monomer to enhance the cell adhesion onto the particles Moreover chitosan was coated on poly(HEMA) microparticles to increase the biocompatibility of the particles To promote the intermolecular forces onto cells porous poly(HEMA) microparticles with average diameter of 400 ?m were successfully synthesized For comparison the average diameter around 650 nm poly(HEMA) nanoparticles were also synthesized Adding of DEAEMA monomer in poly(HEMA) microparticles enhanced the thermal stability of the synthesized particles From the thermogravimetric analysis the temperatures of 5% weight loss (T5%) for poly(HEMA) microparticles without and with DEAEMA were estimated as 204℃ and 275℃ respectively The synthesized cationic charged monomer consisted in poly(HEMA) microparticles promoted the L929 cell adhesion clearly Chitosan coated poly(HEMA) microparticles also increased the attached cell amounts of L929 significantly The optimal cell adhesion was achieved on the poly(HEMA) microparticles with 0 02 mol DEAEMA and chitosan coating In this study cell culture using poly(HEMA) nanoparticles was also carried out The results showed that poly(HEMA) nanoparticles adhesion on L929 cells was too weak to attach onto cells From the results the synthesized poly(HEMA) porous microparticles coated with chitosan showed a good future for 3-D tissue engineering application using as scaffolds

Date of Award	2020
Original language	English
Supervisor	Jui-Hsiang Liu (Supervisor)