Functional characterization of PeMADS28 a B-sister MADS-box gene from Phalaenopsis equestris

  • 沈 青玉

Student thesis: Master's Thesis


The ovules and the egg cells are well-development to be fertilized at anthesis in most of flowering plants In contrast ovule development is triggered by pollination in Phalaenopsis orchids During ?ower evolution expression pattern of B-sister genes maintained in female reproductive organs Moreover B-sister genes may serve as ‘marker genes’ for the development of (inner) integument tissues which are the phylogenetically ‘oldest’ structures surrounding the female gametophyte of seed plants In the genetics studies of ovule development ARABIDOPSIS BSISTER (ABS) gene was expressed mainly in the ovule In petunia FLORAL BINDING PROTEIN24 (FBP24) was necessary to determine the identity of the endothelial layer of the ovule Moreover OsMADS29 is a key regulator of early rice seed development in previous studies In this study PeMADS28 a B-sister gene was cloned and characterized from the Phalaenopsis equestris The results of RT-PCR and quantitative real-time RT-PCR showed that the PeMADS28 gene expression was first detected at the 32 day after pollination (DAP) and sustained to 48 DAP which associated with the inner and the outer integuments development Analysis of protein-protein interactions among PeMADS28 and C- D- E-class proteins were examined by using BiFC assay Results revealed that PeMADS28 could form homodimers in the nucleus and interact with PeSEP3 and PeMADS7 respectively However PeMADS28 could not form heterodimers with PeMADS1 In addition ectopic expression in Arabidopsis thaliana induced curled and small rosette leaves in early development stage In addition the silique length is shorter compared to that of wild-type These ?ndings suggest the important role of PeMADS28 in Phalaenopsis ovule development and provide insights into the ancestral function of B-sister genes
Date of Award2015 Aug 12
Original languageEnglish
SupervisorWen-Chieh Tsai (Supervisor)

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