Functional recoveries of sciatic nerve regeneration by combining chitosan-coated conduit and neurosphere cells induced from adipose-derived stem cells

  • 薛 元毓

Student thesis: Doctoral Thesis


Peripheral nerve injuries are a significant source of disability Although adherence to well-established basic principles of evaluation and repair can optimize results clinical outcomes of peripheral nerve injuries are often suboptimal The stem cell therapy of neural progenitor cell (NPC) from adipose-derived stem cell (ASC) may bring hope to peripheral nerve injury especially in the clinical reality of tissue shortage Transdifferentiation can be induced from ASC to NPC by adding numerous neurotrophic factors or by mechanical microenvironment such as chitosan surface coating To our knowledge chitosan has widely been used for drug release and 3-dimentional scaffold for tissue engineering And owning to the characteristics of biodegradable and biocompatible in nature it also applies as nerve conduit to guide nerve regeneration In author’s previous study transdifferentiation of ASC into NPC can be achieved by using chitosan surface coating as the 3-D scaffold Under the consideration of slow nature of nerve regeneration continued maintenance of the microenvironment for the NPC seems possible to achieve by combined use of the chitosan conduit with induced NPC from ASC The purpose of this study is to use the in vivo rat model of sciatic nerve transection to survey the therapeutic benefit of chitosan coated conduit containing with induced NPC from ASC For the analysis of functional recoveries we will focus on the improvement of the walking track analysis relative gastrocnemius muscle weight measurement and the ratio of regenerated myelinated axons For the exploration of underlying mechanism we will try to investigate the survival of functional cells after treatment including the inflammatory profile
Date of Award2015 Feb 2
Original languageEnglish
SupervisorChia-Ching Wu (Supervisor)

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