High-fold Sample Preconcentration on a Portable Paper-based Device

Abstract

Microfluidic paper-based analytical devices (?PADs) are rapidly becoming as one of importan medical analyses and environment inspections for enhancing human life Nevertheless there are still lots of issues and applications for proving the worth of the ?PADs In this thesis we emerged two to three convergent channels to one by focusing concept The concentration can be raised the factor from 20-fold to over 140-fold by using the triple channels This means we are not just can improve three times than the original channel according to the focusing concept we can reach seven times improvement than before just use three times volume of sample from the initial concentration of 10e-005 M under 115 V Moreover we are the first to develop the high-fold portable preconcentrator device called CHAPPIE (Cheap and Handy Analysis of Portable Preconcentrator included Isolated Electricity) that not only can supply 115 V just use batteries but also can do the process anywhere especially those poor resources setting regions Finally we also use fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) for displaying that proteins in diluted solution can be concentrated by a factor of 100 from the initial concentration of 10e-005 M under the application of 115 V in CHAPPIE for practical applications Furthermore we are the first to measure the temperature on the ?PAD This facile experiment proves that the Joule heating on the ?PADs is minor

Date of Award	2015 Aug 17
Original language	English
Supervisor	Ruey-Jen Yang (Supervisor)