HLH-30/TFEB-mediated autophagy is required for bacterial pore-forming toxin defense in Caenorhabditis elegans

  • 陳 奐達

Student thesis: Doctoral Thesis

Abstract

Autophagy is an evolutionarily conserved intracellular system that maintains cellular homeostasis by degrading and recycling damaged cellular components through the autophagosomal-lysosomal pathway Nowadays research indicates autophagy plays important roles in various mechanisms such as embryogenesis and cell differentiation cancer degenerative neuron diseases bacterial and viral infection The transcription factor HLH-30/TFEB-mediated autophagy has been reported to regulate lysosomal biogenesis and aging through autophagy Furthermore HLH-30 is also involved in modulating the tolerance to bacterial infection through autophagy but less is known about the bona fide bacterial virulent factor that activates HLH-30 and autophagy Here we unveil that bacterial membrane pore-forming toxin (PFT) induces autophagy in an HLH-30-dependent manner in Caenorhabditis elegans Moreover autophagy controls the susceptibility of animals to PFT toxicity through xenophagic degradation of PFT and contributes to the repair of membrane-pore autonomously in the PFT-targeted intestinal cells in C elegans These results demonstrate that autophagy is induced partly at the transcriptional level through HLH-30 activation and is required to protect metazoan upon PFT intoxication Together our data show a new insight between HLH-30-mediated autophagy and epithelium intrinsic cellular defense against the single most common mode of bacterialvirulent factor attack in vivo
Date of Award2017 Feb 6
Original languageEnglish
SupervisorChang-Shi Chen (Supervisor)

Cite this

HLH-30/TFEB-mediated autophagy is required for bacterial pore-forming toxin defense in Caenorhabditis elegans
奐達, 陳. (Author). 2017 Feb 6

Student thesis: Doctoral Thesis