In Situ Investigation of Molecular Immobilization on Au Surface by Surface-Enhanced Infrared Absorption Spectroscopy

  • 吳 宜榛

Student thesis: Master's Thesis

Abstract

Covalent binding has been wildly used in biosensors and biomaterials With the vigorous development of biosensors covalent immobilization is getting more and more attention The process of forming covalent bonds on biosensors including: (1) forming a self-assembly monolayer (SAM) (2) activating the SAM with crosslinkers to form an active layer and then (3) covalent attachment of biomolecules However the immobilization parameters are still based on experience and vary from one study to another In this study 11-mercaptoundecanoic acid (MUA) was used to form SAM on gold surface activating the surface through EDC/NHS and then formed peptide with aminoferrocene (AmFc) Known for high sensitivity low background interference and in-situ observation attenuated total reflection surface enhanced infrared adsorption spectroscopy (ATR-SEIRAS) was used to investigate the immobilization process and then to find a relative well optimized parameters
Date of Award2016 Aug 11
Original languageEnglish
SupervisorHsien-Chang Chang (Supervisor)

Cite this

In Situ Investigation of Molecular Immobilization on Au Surface by Surface-Enhanced Infrared Absorption Spectroscopy
宜榛, 吳. (Author). 2016 Aug 11

Student thesis: Master's Thesis