Modulation and Characterization of Nuclear Factor κB Inhibitor-alpha (IκB?) for Environmental Stress in Orange-spotted Grouper (Epinephelus coioides)

  • 謝 季妤

Student thesis: Master's Thesis

Abstract

One of the important transcription factors in innate immunity NF-κB/IκB? complex which can translocate to nucleus to bind to the promoter and regulate pro-inflammatory cytokine gene to modulate immune responses and defeat pathogens protect fish from pathogens infection in water even without a sound adaptive immunity like mammals In this study the open reading frame cDNA of orange-spotted grouper (Epinephelus coioides) IκB? were cloned with 924 bp and encoded 308 amino acids From the real-time PCR analysis it showed that NF-κB and IκB? widely expressed in various organs while under nervous necrosis virus (NNV) infection they mainly expressed in brain head kidney and liver; however dissociated IκB? protein expressed in immune organs under NNV infection On the other hand the expression of p65 and p50 showed 9 6-fold and 6 2-fold up-regulated at short time and gradually down-regulated under low temperature while gradually up-regulated under high temperature However under high temperature as well as NNV-infected NF-κB and IκB? revealed delayed up-regulation compared to non-infected group Taken together in orange-spotted grouper NF-κB/IκB? complex regulates gene changes under NNV infection or changes in environmental temperature which in turn may inhibit excessive inflammatory responses and increase the survival of grouper Therefore the NF-κB/IκB? complex may play a significantly regulatory role in the innate immune mechanism of grouper against environmental stress
Date of Award2018 Dec 28
Original languageEnglish
SupervisorTzong-Yueh Chen (Supervisor)

Cite this

Modulation and Characterization of Nuclear Factor κB Inhibitor-alpha (IκB?) for Environmental Stress in Orange-spotted Grouper (Epinephelus coioides)
季妤, 謝. (Author). 2018 Dec 28

Student thesis: Master's Thesis