Physicochemical properties and vitamin E acetate encapsulation efficiency of catanionic vesicles fabricated from triple-chained ion pair amphiphiles

Abstract

In this study two triple-chained ion pair amphiphiles dihexadecyldimethylammonium-hexadecylsulfate (DHDA-HS) and dihexadecyldimethylammonium-dodecylsulfate (DHDA-DS) were prepared from double-chain cationic surfactant dihexadecyldimethylammonium bromide (DHDAB) with single-chain anionic surfactants sodium hexadecylsulfate (SHS) and sodium dodecylsulfate (SDS) With the consideration of drug delivery applications dihexadecyl phosphate (DHDP) was added into DHDA-HS and DHDA-DS respectively First DHDA-HS can be fabricated as positively charged catanionic vesicles with the size of 130nm With addition of DHDP the size of DHDA-HS vesicle turned from 130nm into 100nm with negative charge In addition DHDA-HS/DHDP vesicle had high physical stability Membrane characteristic was analyzed by fluorescence polarization and infrared spectroscopy Infrared spectroscopy analysis revealed that increasing content of DHDP causes the arrangement of hydrocarbon chain more disordered and fluorescence polarization analysis indicated the phase transition temperature was decreased On drug encapsulation DHDA-HS/DHDP vesicle and DHDA-DS/DHDP vesicle both could encapsulate hydrophobic drug vitamin E acetate efficiently In addition with the successful encapsulation of hydrophilic drug arbutin it was proved that the DHDA-HS/DHDP and DHDA-DS/DHDP vesicles indeed possessed vesicular structures BEAS-2B cells and H1299 cells were used to conduct the cytotoxicity assay for the vitamin E acetate encapsulated vesicles The results indicated that the increased content of vitamin E acetate reduced the cytotoxicity and the hemolysis test also showed the vitamin E acetate encapsulation could cause hemolysis ratio decrease

Date of Award	2019
Original language	English
Supervisor	Chien-Hsiang Chang (Supervisor)