Quantitative Analysis Based on Impedance Measurement for Human Chorionic Gonadotropin

Abstract

This study a novel electrode biochip for immunoassay was applied to detect sandwich-immunoassay of human chorionic gonadotropin a gold nanoparticles (AuNPs) as a label of antibody to enhance immunoassay impedance quantitative analysis detection Compared with traditional enzyme-linked immunosorbent assay (ELISA) we utilized the LCR meter instrument to detect the electric signal on electrode biochip By detecting the impedance changes because of sandwich-immunoassay reaction a sandwich-immunoassay detection system was established In order to avoid a gap between a nitrocellulose membrane and electrode bringing about the unstable electric signal a nitrocellulose membrane was fixed on a surface of electrode chip In the study combined impedance changing detection and gold nanoparticles labelled in sandwich-immunoassay achieves quantitative the concentration of human chorionic gonadotropin(hCG) antigen The results show that the cut-off value decreased from 25 mIU/mL to 6 25 mIU/mL The coefficient of determination between electric signal and concentration of hCG has come to 0 9899

Date of Award	2019
Original language	English
Supervisor	Yu-Cheng Lin (Supervisor)