Rapid Microfluidic Paper-Based Chip for Phosphorus Detection in Serum

Abstract

Chronic diseases become more prevalent because of population ageing In Taiwan more and more people suffer the chronic kidney disease (CKD) CKD patients cannot maintain phosphorus balance due to kidney damage which may cause hyperphosphatemia Currently there are no commercial products such as glucose meters can help CKD patients perform self-tests This study develops a fast detection system to help them detect phosphorus concentration in their blood immediately The concept of Lab-on-a-chip (LOC) and Point-of-care-testing (POCT) are combined to develop a paper-based chip for phosphorus detection in serum In the proposed method samples are dropped into the reaction area of the paper-based chip After the colorimetric reaction the Red Green Blue (RGB) value is read by the paper-based chip detection system and phosphorus concentration is deduced from the RGB value The experimental results indicate that the R+G+B value linearity can approximate 0 99 in the range of phosphorus concentration 1~9 mg/ dL In order to verify the application of the paper-based chip with real samples 100 serum samples from CKD patients are tested The result shows that the recovery rate of 95 samples was between 100 ± 15% verify the feasibility of the paper-based chip Overall the results show that the proposed paper-based chip provides a rapid low-cost and reliable approach for phosphorus detection in serum

Date of Award	2020
Original language	English
Supervisor	Lung-Ming Fu (Supervisor)