Shear stress and erythromycin stimulations in endothelial cells for treatment of neonatal hypoxic-ischemia brain injury

  • 江 宜倫

Student thesis: Master's Thesis

Abstract

Stem cells can differentiate to endothelial lineage as a source for tissue engineering of vessels However the rarity of cell source and low differentiation efficiency are the main limitation for stem cell therapy in vessel regeneration Our previously study demonstrated a successful endothelial cell (EC) differentiation by integration of biomechanical force and chemical factors but the mechanism is still unknown Furthermore we build up a cell therapy of brain hypoxic-ischemia (HI) model by using human umbilical vein endothelial cells (HUVECs) The differentiated cells will apply to this animal model in the future By static HUVECs transplantation in brain HI model brain injury and cell death were decreased Laminar shear stress (LSS)-treated HUVECs group in animal model had not significantly repair function than static HUVECs Previously research indicates that CXCR4 a chemoattractant receptor is reduced by LSS One paper showed that erythromycin (EM) increased CXCR4 expression Therefore we combined LSS with EM to stimulate HUVECs We found that EM treatment after LSS stimulation of HUVEC transplantation was useful for rescuing brain injury In this study we built up successful cell therapy for brain injury model Furthermore repair function of ECs can be enlarged by increasing EM-induced CXCR4 expression
Date of Award2017 Feb 18
Original languageEnglish
SupervisorChia-Ching Wu (Supervisor)

Cite this

Shear stress and erythromycin stimulations in endothelial cells for treatment of neonatal hypoxic-ischemia brain injury
宜倫, 江. (Author). 2017 Feb 18

Student thesis: Master's Thesis