Stimulation of wound healing process through ROS/RNS signals indirectly generated by N2/Ar micro-plasma - in vitro and in vivo studies

Abstract

In this work non-thermal N2/Ar micro-plasma was applied to fibroblast cells and second degree burn in mice to investigate the bio-safety and bio-efficiency of micro-plasma device for wound healing process With the main parameters are currently chosen by adding 0 5% N2 in argon plasma and supplying RF power of 17 W is first applied to fibroblast cells and induced the biological effect in vitro study The cells number increased three folds for plasma exposure time of 5 or 10 sec followed by cell culture for 48 hrs The cell coverage rate rose 20% for the same plasma exposure time followed by cell culture for 6 or 12 hrs 0 5% N2/Ar micro-plasma exposure can particularly be used to achieve the stimulated release of FGF7 and subsequent enhancement of cells proliferation and migration A possible mechanism for re-epithelialization at the molecular level will be proposed based on the results of this work Based on the results in vitro study N2/Ar micro-plasma with supplying power of 13 W was applied to improve healing on the second degree burn wound mice followed by repairing tissue assessments ROS/RNS signals from the increase of ROS/RNS concentration around the plasma-exposed wound bed are most probably correlated with the angiogenesis and epithelialization processes From this animal model non-thermal N2/Ar micro-plasma is presumably effective for the stimulation of burn wound healing on mice a possible in vivo mechanism for the enhanced angiogenesis and epithelialization processes in the plasma-exposed mice is thereafter proposed

Date of Award	2014 Nov 24
Original language	English
Supervisor	Jiunn-Der Liao (Supervisor)