Studies of biocompatible polymer surface modification combined with physical stimulation for osteoblast-like cells and adipose-derived stem cells proliferation and differentiation

Abstract

SUMMARY A mild and stable process for PDMS surface modification and a bio-mimicking dynamic culturing platform were developed to evaluate the cellular responses of osteoblast-like MG-63 cells on PDMS substrate with different hydrophilic grafting layer The experimental platform developed here including the dynamic culture system and the modification scheme for forming PDMS-COLL could be beneficial in evaluating the cellular responses in a more bio-mimicking and clinically relevant manner as well as in cultivating the cell/tissue needed for clinical applications The growth factor TGF-β1 was co-grafted with the type I collagen onto the elastic PDMS substrate for examining the combinatory effect of surface chemistry and mechanical stimuli on rADSCs’ chondrogenesis differentiation After 72hr static culture the PDMS-COLL and PDMSCOLL/TGF-β1 were shown to be effective to induce the chondrogenic differentiation than control group In contrast if the dynamic stretching stimuli were utilized the efficiency in chondrogenic differentiation is dependent upon the substrate as well as the dynamic stretching protocol utilized The surface grafted with collagen and TGF-β1 layer induced more proteoglycan secretion than the one with collagen only For PU scaffold applications the extracellular matrix-like type I collagen layer and the grafted antioxidative reagent NAC have improved the rADSCs adherence and enhanced the stem cell proliferation efficiency in vitro Further the use of dynamic compression stimulation has promoted rADSCs differentiation into the chondrogenic lineage within a short cell culture duration This anti-oxidative PU scaffold in the combination of physical compression stimulation could become a novel approach for stem cell therapy in the future

Date of Award	2021
Original language	English
Supervisor	Jui-Che Lin (Supervisor)