Study of the Non-Thermal Micro Plasma on the Co-culture of Melanoma Cells and Fibroblasts

Abstract

In this study the co-culture system of melanoma cell B16-F10 and fibroblasts L929 was used to investigate the selective effect of non-thermal micro plasma (NTP) At first to find the best helium flow rate for experiment the plasma temperature and generated species intensity were measured under different He flow rate and 1 6 SLM of He was proper for the further experiment The cell viability assay was separated to mono- and co-culture system to see the cell-cell interaction in co-culture system after NTP treatment The results showed that co-cultured B16-F10 was more resistant to NTP compared with mono-cultured B16-F10 and it could be due to the cytokines release in the co-culture system In general NTP selectively induce B16-F10 rather than L929 in both culture systems for low dose plasma (30-90 s); however both cells in co-culture system encountered large toxicity for high dose plasma (180 s) leading to around 50-60 % of cell death Therefore the double low dose treatment was replaced with single high dose treatment to keep higher living normal cell rate and inhibit cancer cell growth as usual The results showed that in the same total treatment time double plasma treatment enhanced around 20 % of B16-F10 apoptosis compared with single treatment and leaving around 80-90 % of L929 alive

Date of Award	2019
Original language	English
Supervisor	Jiunn-Der Liao (Supervisor)